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M9470130.TXT
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1994-07-02
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Document 0130
DOCN M9470130
TI HIV-1 reactivation after an oxidative stress mediated by different
reactive oxygen species.
DT 9409
AU Piette J; Legrand-Poels S; Laboratory of Fundamental Virology,
University of Liege,; Belgium.
SO Chem Biol Interact. 1994 Jun;91(2-3):79-89. Unique Identifier : AIDSLINE
MED/94251850
AB An important aspect of infection by the human immunodeficiency virus
(HIV-1) type 1 is its long clinical latency period, suggesting that the
provirus may remain latent for extended periods of time after primary
infection. Numerous factors such as cytokines, tumor promoters,
co-infection by several viruses and physical agents are able to
reactivate latent virus. Since a common denominator, shared by several
of these agents, is their ability to cause stress conditions, we have
examined the effects of an oxidative stress mediated by reactive oxygen
species on HIV-1 latently infected monocytes (U1) or lymphocytes
(ACH-2). Exposure of these two cell lines to hydrogen peroxide causes a
decrease of cell viability but among the cells surviving the treatment,
a HIV-1 reactivation can be observed as measured by increased RT
activities depicted in cell supernatants or by the appearance of HIV-1
antigens inside cells. Singlet oxygen (1O2) when generated either in the
cytoplasm or in the cell nucleus can also promote an important HIV-1
reactivation from treated cells. However, extracellular generation of
1O2 cannot trigger the HIV-1 reactivation although this kind of
treatment is highly cytotoxic. These experiments demonstrate that
different reactive oxygen species are able to lead to an intracellular
pro-oxidant state initiating one or several signalling pathways which
lead in fine to the HIV-1 LTR transactivation by regulatory proteins.
DE Cell Line Cell Survival/DRUG EFFECTS Human Hydrogen
Peroxide/PHARMACOLOGY HIV-1/DRUG EFFECTS/*PHYSIOLOGY Monocytes/DRUG
EFFECTS/*MICROBIOLOGY Oxidation-Reduction Oxygen/PHARMACOLOGY
Reactive Oxygen Species/*PHARMACOLOGY Support, Non-U.S. Gov't
T-Lymphocytes/DRUG EFFECTS/*MICROBIOLOGY *Virus Activation *Virus
Latency JOURNAL ARTICLE REVIEW REVIEW, TUTORIAL
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).